Mama Ji's Molecular Kitchen

skip links

HomeAlkaline lysisChromatographySDS-PAGEAgarose gelPCRSouthern blotNorthern blotWestern blotRadio immune precipitationRestriction enzymesPlasmidsSequencing

ProtocolsThanksReferencesemail

Education accessible logo

Welcome to Mama Ji's Molecular Kitchen!

I developed this site as a way of avoiding the traditional thesis project for my master's degree. Rather than have a paper that would collect dust somewhere, I wanted to produce something people would use all the time. Please use this site and tell others about it!

 
 

Prep & Purification

Electro-
phoresis

Visualization, Detection & Quantitation

Recombination

Characterization

DNA Techniques

Alkaline Lysis

SDS-Page

Agarose Gel

PCR

Southern Blot

Restriction Enzymes

Plasmids

Sequencing

RNA Techniques

. .

Northern Blot

RT-PCR

. .

Protein Techniques

Chroma-
tography

.

Western Blot

Radio-Immune Precipitation

. .

There are lots of sites out there full of molecular techniques and protocols, but very little information (either online or in readable hardcopy form) that explain how and why these techniques work. That's where this site comes in. I hope you will learn as much from it and enjoy it as much as I have. I've written in simple terms about a number of commonly used molecular techniques, explaining what they are and how they work. So bookmark this site and read it the next time a professor or colleague describes something in technical jargon you can't decipher or when a high-profile court trial makes molecular sciences an interest to the general public. And if you need an expert witness, hire me. I work cheap and cleanup real good.

Use the chart above to find a subject and click away. If you have any suggestions for improvements or would like to see a technique added, please email me.

Also feel free to email if you'd like to make your own contribution to this site. Lastly, I've included a bunch of links to sites that have specific protocols so you can take a look at recipes that have worked for other scientists.

Guruatma "Ji" Khalsa, M.N.S.