Enzyme: a protein that changes the speed of chemical reactions.
Extract: to draw or pull something out (especially from another material).
Indiana Jane must have permission to work with each bone. When she is ready to extract DNA, she cuts a small piece of bone or tooth and crushes it into powder. It looks a little like flour you can bake with.
In the bone powder are millions of bone cells and millions of cells from other organisms, including bacteria and parasites. A copy of the DNA is located inside every single cell.
Next, she needs to crack open the cell walls so that the DNA spills out. A special enzyme does this job. It is a lot like cracking open an egg into a bowl, except that it is all microscopic.
But DNA is not the only thing inside a cell. When the cell walls are cracked, other cell parts come out as well. To separate the DNA from the other parts of the cell, the next step is the cleaning step. Indiana Jane must capture only the DNA but leave all of the “dirt” behind.
When the DNA is clean, it is ready for analysis. Indiana Jane hopes we have captured more than just human DNA. This is where she hopes the microbe DNA will be.
Skull section image via Wikimedia Commons by Phyzome.
Kelly Harkins. (2014, June 02). The Hunt for DNA. ASU - Ask A Biologist. Retrieved March 18, 2023 from https://askabiologist.asu.edu/hunt-dna
Kelly Harkins. "The Hunt for DNA". ASU - Ask A Biologist. 02 June, 2014. https://askabiologist.asu.edu/hunt-dna
Kelly Harkins. "The Hunt for DNA". ASU - Ask A Biologist. 02 Jun 2014. ASU - Ask A Biologist, Web. 18 Mar 2023. https://askabiologist.asu.edu/hunt-dna
Skulls and other bones can teach us a lot more about our ancestors than you might think.
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